Zakład Genomiki I Bioróżnorodności

SCIENTIFIC ACHIEVEMENTS

 

1. Identification of genetic markers on the chicken chromosome 4, in GGA4q11-12 (MCW0114 and ADL0241) and GGA4q21-22 (MCW0170) regions, and physical mapping markers significantly linked with the QTL for the thickness of the egg shell [Sazanov, Jaszczak et al., 2005 - Animal Genetics 36, 161-163].
2. Identification of genetic background of susceptibility to stress related to delta opioid receptor gene C320T polymorphism, also proved to be relevant with stress dependent analgesia in mouse [Sacharczuk et al., 2010 - Pain 149, 506-513].
3. Showing stability of expression of housekeeping genes TBP, TOP2B and HPRT1 in the liver tissue and muscle of pigs, that can be used as reference genes for standardization of the transcriptomic results [Pierzchała et al., 2011 - Animal Science Papers and Reports 29, 53-63].
4. Identification of differences in the transcriptional profile of genes encoding GHR, IGF1R, IGF1 and IGF2 in the liver of pigs as related to pig breed. Showing a correlation between the level of transcription of IGF1R and IGF2 genes and pig carcass leanness/fatness [Pierzchała et al., 2011 - Molecular Biology Reports 31, 3802-3804].
5. Showing the effect  of pigs, age and breed on the transcription levels of genes belonging to the myogenin family (MYF5 and MYF6) in different muscles [Pierzchała et al., 2011 - Animal Science Papers and Reports 29, 231-246].
6. Showing associations between polymorphisms of c-Ski, c-myc, DGAT1, AGL, cystatin B and calpastatin genes and selected carcass quality traits of pigs [Urbański et al., 2010 - Tieraerztliche Umschau 65, 480].
7. Characterization  of new microsatellite loci that can be used as markers to create comprehensive genetic map of the ostrich, also useful a as genetic marker for the laying performance traits in ostrich [Kawka, Horbańczuk et al., 2012 - Molecular Biology Reports 39, 3369-3374; Kawka, Horbańczuk, Pierzchała et al., 2012 - Molecular Biology Reports DOI: 10.1007/s11033 -012-1632-x].
8. The significant correlations between polymorphisms in the EGF, AREG and OPN genes and  their transcription levels in the reproductive tract of pigs (uterus, oviduct and ovary) and the value of the studied traits associated with reproduction of sows were found [Goluch, Korwin-Kossakowska et al., 2009 – Neuroendocrinology Letters  30, 253-257].
9.  In international cooperation within the Q-PorkChains project (6th EU Framework Programme), together with the Department of Molecular Cytogenetics, the association  of expression of VAMP5, ACAA2, CYTB, GLOD4 genes in muscle tissue with pig meat quality traits has been found, which indicates the possibility of their use as biomarkers of quality and technological suitability of pork.
10.  The results of production, reproduction rates and the health status of Japanese quail, fed with one of three types of feed: non-GMO, the GMO soybeans (Roundup Ready) and GMO maize (MON810) were compared. After examination of four generations of birds no negative effect of the use of GMO soya or maize was found with regard to performance traits [Sartowska, Korwin-Kossakowska et al., 2012 - Archiv für Geflügekunde 76, 140 144]. In none of the examined tissues (liver, gizzard, heart, breast muscle or splean), nor in eggs was found a gene construct derived from genetically modified plants [Korwin-Kossakowska, Sartowska et al., 2012 - Journal of Animal and Feed Sciences, submitted].
11. Complete sequence of mitochondrial genome (mtDNA) of Polish Red cattle was identified. The results of dating based on molecular clock indicates that the founder cow of the hitherto unknown in Bostaurus haplogroup T3b had to live around 10,000 years ago, i.e. at the beginning of the “Neolithic Revolution”.
12.  Haplotype diversity in five microsatellite loci of the Y chromosome in bulls of cattle breeds being under the preserved breeding in Poland was identified. Twenty new Y-chromosome haplotypes were identified. Paternal lines identified in native cattle were located in the global phylogenetic tree of the Y-chromosome of domesticated cattle breeds. The resulting distribution of Y-chromosome haplotypes indicates a significant distinctiveness of identified male lines.
13.  In livestock and wildlife species (including the protected species) reference sequences of the mtDNA cytb gene (14897-15170) and the gene of cytochrome oxidase subunit I (COI) have been identified, which can be a useful tool to identify the origin of anonymous biological traces [Prusak, Grzybowski, 2004 – Acta Biochemica Polonica 51, 897-905].
14. The level of genetic diversity (13 STR markers, 4 regions of mtDNA) within and between populations of the European pond turtle in Poland was determined. It was shown that the present Polish territories are inhabited by two indigenous evolutionary distinct lineages of turtles having their origin in two different glacial refuges - the western lineage (IIb) and the eastern one (Ia). Conclusions were presented in the form of guidance useful in the active protection of pond turtles in Poland [Prusak et al., 2011 - Biologia 66, 893-898].